Agrocybe aegerita Serves As a Gateway for Identifying Sesquiterpene Biosynthetic Enzymes in Higher Fungi.
Identifieur interne : 000613 ( Main/Exploration ); précédent : 000612; suivant : 000614Agrocybe aegerita Serves As a Gateway for Identifying Sesquiterpene Biosynthetic Enzymes in Higher Fungi.
Auteurs : Congqiang Zhang ; Xixian Chen ; Axel Orban [Allemagne] ; Sudha Shukal ; Florian Birk [Allemagne] ; Heng-Phon Too [Singapour] ; Martin Rühl [Allemagne]Source :
- ACS chemical biology [ 1554-8937 ] ; 2020.
Abstract
Terpenoids constitute a structurally diverse group of natural products with wide applications in the pharmaceutical, nutritional, flavor and fragrance industries. Fungi are known to produce a large variety of terpenoids, yet fungal terpene synthases remain largely unexploited. Here, we report the sesquiterpene network and gene clusters of the black poplar mushroom Agrocybe aegerita. Among 11 putative sesquiterpene synthases (STSs) identified in its genome, nine are functional, including two novel synthases producing viridiflorol and viridiflorene. On this basis, an additional 1133 STS homologues from higher fungi have been curated and used for a sequence similarity network to probe isofunctional STS groups. With the focus on two STS groups, one producing viridiflorene/viridiflorol and one Δ6-protoilludene, the isofunctionality was probed and verified. Three new Δ6-protoilludene synthases and two new viridflorene/viridiflorol synthases from five different fungi were correctly predicted. The study herein serves as a fundamental predictive framework for the discovery of fungal STSs and biosynthesis of novel terpenoids. Furthermore, it becomes clear that fungal STS function differs between the phyla Ascomycota and Basidiomycota with the latter phylum being more dominant in the overall number and variability. This study aims to encourage the scientific community to further work on fungal STS and the products, biological functions, and potential applications of this vast source of natural products.
DOI: 10.1021/acschembio.0c00155
PubMed: 32233445
Affiliations:
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type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Terpenoids constitute a structurally diverse group of natural products with wide applications in the pharmaceutical, nutritional, flavor and fragrance industries. Fungi are known to produce a large variety of terpenoids, yet fungal terpene synthases remain largely unexploited. Here, we report the sesquiterpene network and gene clusters of the black poplar mushroom <i>Agrocybe aegerita.</i> Among 11 putative sesquiterpene synthases (STSs) identified in its genome, nine are functional, including two novel synthases producing viridiflorol and viridiflorene. On this basis, an additional 1133 STS homologues from higher fungi have been curated and used for a sequence similarity network to probe isofunctional STS groups. With the focus on two STS groups, one producing viridiflorene/viridiflorol and one Δ<sup>6</sup>-protoilludene, the isofunctionality was probed and verified. Three new Δ<sup>6</sup>-protoilludene synthases and two new viridflorene/viridiflorol synthases from five different fungi were correctly predicted. The study herein serves as a fundamental predictive framework for the discovery of fungal STSs and biosynthesis of novel terpenoids. Furthermore, it becomes clear that fungal STS function differs between the phyla Ascomycota and Basidiomycota with the latter phylum being more dominant in the overall number and variability. This study aims to encourage the scientific community to further work on fungal STS and the products, biological functions, and potential applications of this vast source of natural products.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32233445</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1554-8937</ISSN><JournalIssue CitedMedium="Internet"><Volume>15</Volume><Issue>5</Issue><PubDate><Year>2020</Year><Month>05</Month><Day>15</Day></PubDate></JournalIssue><Title>ACS chemical biology</Title><ISOAbbreviation>ACS Chem Biol</ISOAbbreviation></Journal><ArticleTitle><i>Agrocybe aegerita</i> Serves As a Gateway for Identifying Sesquiterpene Biosynthetic Enzymes in Higher Fungi.</ArticleTitle><Pagination><MedlinePgn>1268-1277</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/acschembio.0c00155</ELocationID><Abstract><AbstractText>Terpenoids constitute a structurally diverse group of natural products with wide applications in the pharmaceutical, nutritional, flavor and fragrance industries. Fungi are known to produce a large variety of terpenoids, yet fungal terpene synthases remain largely unexploited. Here, we report the sesquiterpene network and gene clusters of the black poplar mushroom <i>Agrocybe aegerita.</i> Among 11 putative sesquiterpene synthases (STSs) identified in its genome, nine are functional, including two novel synthases producing viridiflorol and viridiflorene. On this basis, an additional 1133 STS homologues from higher fungi have been curated and used for a sequence similarity network to probe isofunctional STS groups. With the focus on two STS groups, one producing viridiflorene/viridiflorol and one Δ<sup>6</sup>-protoilludene, the isofunctionality was probed and verified. Three new Δ<sup>6</sup>-protoilludene synthases and two new viridflorene/viridiflorol synthases from five different fungi were correctly predicted. The study herein serves as a fundamental predictive framework for the discovery of fungal STSs and biosynthesis of novel terpenoids. Furthermore, it becomes clear that fungal STS function differs between the phyla Ascomycota and Basidiomycota with the latter phylum being more dominant in the overall number and variability. This study aims to encourage the scientific community to further work on fungal STS and the products, biological functions, and potential applications of this vast source of natural products.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Congqiang</ForeName><Initials>C</Initials><Identifier Source="ORCID">0000-0003-1070-8806</Identifier><AffiliationInfo><Affiliation>Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore, Republic of Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xixian</ForeName><Initials>X</Initials><Identifier Source="ORCID">0000-0002-0335-2058</Identifier><AffiliationInfo><Affiliation>Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore, Republic of Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Orban</LastName><ForeName>Axel</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shukal</LastName><ForeName>Sudha</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore, Republic of Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Birk</LastName><ForeName>Florian</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Too</LastName><ForeName>Heng-Phon</ForeName><Initials>HP</Initials><Identifier Source="ORCID">0000-0003-0791-3796</Identifier><AffiliationInfo><Affiliation>Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore, Republic of Singapore.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Republic of Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rühl</LastName><ForeName>Martin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Giessen, Germany.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Fraunhofer Institute for Molecular Biology and Applied Ecology, Branch for Bioresources, Giessen, Germany.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ACS Chem Biol</MedlineTA><NlmUniqueID>101282906</NlmUniqueID><ISSNLinking>1554-8929</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32233445</ArticleId><ArticleId IdType="doi">10.1021/acschembio.0c00155</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Singapour</li></country><orgName><li>Université nationale de Singapour</li></orgName></list><tree><noCountry><name sortKey="Chen, Xixian" sort="Chen, Xixian" uniqKey="Chen X" first="Xixian" last="Chen">Xixian Chen</name><name sortKey="Shukal, Sudha" sort="Shukal, Sudha" uniqKey="Shukal S" first="Sudha" last="Shukal">Sudha Shukal</name><name sortKey="Zhang, Congqiang" sort="Zhang, Congqiang" uniqKey="Zhang C" first="Congqiang" last="Zhang">Congqiang Zhang</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Orban, Axel" sort="Orban, Axel" uniqKey="Orban A" first="Axel" last="Orban">Axel Orban</name></noRegion><name sortKey="Birk, Florian" sort="Birk, Florian" uniqKey="Birk F" first="Florian" last="Birk">Florian Birk</name><name sortKey="Ruhl, Martin" sort="Ruhl, Martin" uniqKey="Ruhl M" first="Martin" last="Rühl">Martin Rühl</name><name sortKey="Ruhl, Martin" sort="Ruhl, Martin" uniqKey="Ruhl M" first="Martin" last="Rühl">Martin Rühl</name></country><country name="Singapour"><noRegion><name sortKey="Too, Heng Phon" sort="Too, Heng Phon" uniqKey="Too H" first="Heng-Phon" last="Too">Heng-Phon Too</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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